Treponema pallidum immune adherence test for serodiagnosis of syphilis. 1: An improved method of the TPIA test.

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Treponema pallidum immune adherence test for serodiagnosis of syphilis. 1: An improved method of the TPIA test.

Clinical investigations using the Treponema pallidum immune adherence (TPIA) test as reported by Nelson (1953) were performed only by Olansky et al in 1954. In this paper an improved method of the TPIA test is described. The test was simple to perform and was highly specific for antitreponemal antibody. Furthermore, as it was possible to use heated T. pallidum as antigen the antigen suspension ...

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Treponema pallidum immune adherence test for serodiagnosis of syphilis. 3: Clinical significance and evaluation of treatment.

Antibody titres were measured in patients with clinical syphilis, and the effect of treatment on the results of the Treponema pallidum immune adherence (TPIA) test is reported. In the Treponema pallidum haemagglutination (TPHA) test little change in antibody titre occurred after treatment while in the fluorescent treponemal antibody-absorbed (FTA-ABS) test only a slight decrease occurred. The d...

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Treponema pallidum specific IgM haemagglutination test for serodiagnosis of syphilis.

The Treponema pallidum specific IgM haemagglutination (TP-IgM-HA) test uses erythrocytes sensitised with antiserum to human IgM to separate IgM from IgG in serum. Specific antitreponemal IgM captured in this way is detected by adding a second reagent comprising erythrocytes sensitised with T pallidum antigen. Eighty two serum samples from 82 patients with untreated syphilis, 521 samples from 73...

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Treponema pallidum immune adherence test for serodiagnosis of syphilis. 2: Comparison with glass plate, TPHA, and FTA-ABS tests.

Using sera from 340 patients with syphilis the Treponema pallidum immune adherence (TPIA) test was compared with the glass plate, T. pallidum haemagglutination (TPHA), and fluorescent treponemal antibody-absorbed (FTA-ABS) tests. The results of the TPIA test agreed with those of the glass plate, TPHA, and FTA-ABS tests in 65%, 82%, and 73% of cases respectively. In the quantitative TPIA test no...

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Evaluation of Recombinant Protein TpF 1 of Treponema pallidum for 1 Serodiagnosis of Syphilis

24 Syphilis is a chronic infection caused by Treponema pallidum subsp. pallidum, and 25 the diagnosis of syphilis with sensitive and specific methods is challenging and 26 important for the prevention and treatment of syphilis. In present study, we 27 established a recombinant protein TpF1-based indirect immunoglobulin G (IgG) 28 enzyme-linked immunosorbent assay (ELISA) and Western blot assay ...

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ژورنال

عنوان ژورنال: Sexually Transmitted Infections

سال: 1978

ISSN: 1368-4973

DOI: 10.1136/sti.54.6.369